Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 3, Pages 349-355Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.04.008
Keywords
hybridization biosensor; 2-nitroacridone; DNA; Chronic Myelogenous Leukemia
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Funding
- National Natural Science Foundation of China [20735002, 20575011, 20675015]
- Natural Science Foundation of Fujian Province [200610016]
- Foundation of Fujian Education Department [2005K051]
- Foundation of Fujian Health Department [2005123]
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A new acridone derivative 2-nitroacridone (NAD) was synthesized in this paper, and it was found that NAD had excellent electrochemical activity on the glassy carbon electrode (GCE) with a couple reversible redox peaks at 0.051 V and 0.103 V, respectively. Voltammetry was used to investigate the electrochemical behavior of NAD and the interaction between NAD and salmon sperm DNA. In pH 4.0 phosphate buffer solution, the binding ratio between NAD and salmon sperm DNA was calculated to be 2:1 and the binding constant was 3.19 x 10(5) L/mol. A Chronic Myelogenous Leukemia (CML, Type b(3)a(2)) DNA biosensor was developed by immobilizing covalently single-stranded CML DNA fragments to a modified GCE. The surface hybridization of the immobilized single-stranded CML DNA fragment with its complementary DNA fragment was evidenced by electrochemical methods using NAD as a novel electrochemical indicator, with a detection limit of 6.7 x 10(-9) M and a linear response range of 1.8 x 10(-8) M to 9.1 x 10(-8) M for CML DNA. Selective determination of complementary ssDNA was achieved using differential pulse voltammetry (DPV). (C) 2008 Elsevier B.V. All rights reserved.
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