Journal
BIOSENSORS & BIOELECTRONICS
Volume 23, Issue 8, Pages 1298-1302Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.11.023
Keywords
nanoparticle; array; biosensor; label-free detection; light scattering; antibody-antigen binding
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Funding
- Engineering and Physical Sciences Research Council [EP/C52389X/1] Funding Source: researchfish
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Gold nanoparticles grown in situ from printed seed particles on a glass substrate have been fabricated into a biosensor array. The light-scattering properties of the resulting surfaces show sensitivity to changes in the local refractive index. Each array spot is functionalized with fibrinogen or bovine serum albumin and scattered radiation is used to monitor the refractive index change on label-free binding of the antibodies to their antigens from whole blood antiserum. Data were collected real-time and the association rate constants for the specific antibody-antigen binding were derived from a kinetic analysis. The minimum antibody concentration detection sensitivity is of 100 nM. (c) 2007 Elsevier B.V. All rights reserved.
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