Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 78, Issue 1, Pages 14-18Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.877826
Keywords
polar lipid profiling; Euglena gracilis; LipidBlast; LC-MS/MS
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Funding
- Strategic International Cooperative Program (SICP) - Joint Research Type Japanese (JST) - US (NSF) Joint Research Metabolomics for a low carbon society (METABOLOMICS)
- US National Science Foundation [MCB 1139644]
- US National Institutes of Health [P20 HL113452, U24 DK097154]
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A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.
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