Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 78, Issue 11, Pages 1817-1824Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.942251
Keywords
lipopolysaccharide; antibodies; cholera; vaccine; vibriocidal
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Funding
- Slovak Grant Agency VEGA [2/0026/13]
- Research and Development Operational Programme - ERDF [ITMS 26240120031]
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A glycoconjugate construct was based on attachment of V. cholerae O139 hydrazine-treated lipopolysaccharide (LPS) to carboxylated bovine serum albumin (CBSA) via its amino group. The immunological properties of the glycoconjugate were tested using BALB/c mice, injected subcutaneously without any adjuvant three times at 2 weeks interval. The immunogenicity of the conjugate was estimated by enzyme-linked immunosorbent assay, testing of anti-LPS IgG, IgM, and IgA antibodies. The conjugate elicited a statistically significant increase of LPS-specific IgG levels in mice (p < 0.001). The specific anti-LPS IgG and IgA response after the second booster dose was significantly higher compared with reference and unconjugated detoxified LPS response. Antibodies elicited by the dLPS-CBSA conjugate were vibriocidal.
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