Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 77, Issue 9, Pages 1939-1941Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.130388
Keywords
phospholipase D; hydrolytic reaction; microaqueous water-immiscible organic solvent; phosphatidylserine; transphosphatidylation rate
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Funding
- National Basic Research Program of China [2011CBA00804]
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Phospholipase D (PLD)-mediated transphosphatidylation of phosphatidylcholine (PC) in a biphasic system was limited by the hydrolysis reaction. A biphasic system can produce a large amount of water. To solve this problem, a microaqueous water-immiscible organic solvent was used for the first time in the bioconversion of phosphatidylserine (PS). The transphosphatidylation among 40 mu mol PC, 800 mu mol L-serine, and 0.17 U/mL PLD in 2.133 mL of butyl acetate with 6.25% water (V/V) was conducted at a trans-phosphatidylation rate of 88% (mol/mol), and no hydrolytic reaction was observed. Compared to commonly used biphasic systems, this system shows a similar transphosphatidylation rate, whereas the undesirable hydrolysis of phospholipids was completely suppressed.
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