Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 77, Issue 8, Pages 1655-1660Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.130186
Keywords
acrolein; NADPH-dependent acrolein reduction (NAR); photosynthesis; plant diabetes; reactive carbonyls (RCs)
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Funding
- Japan Society for the Promotion of Science [21570041]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [22114512, 21114006]
- Grants-in-Aid for Scientific Research [22114512, 21114006] Funding Source: KAKEN
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In this study, we sought to determine whether and how an alpha,beta-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200 mu M acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2-dependent O-2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200 mu M acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.
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