Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 76, Issue 12, Pages 2342-2346Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120568
Keywords
starfish; Asterina pectinifera; cathepsin L; recombinant protein; zymography
Categories
Funding
- Pukyong National University [PK-2010-0179]
Ask authors/readers for more resources
To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The promature enzyme of ApCtL, proApCtL, was expressed in Escherichia con, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available