Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 75, Issue 9, Pages 1848-1852Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.110331
Keywords
Arabidopsis thaliana; bimolecular fluorescence complementation (BiFC); Sec13; Sec31; vesicle transport
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [21570046]
- Grants-in-Aid for Scientific Research [21570046] Funding Source: KAKEN
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COPII vesicles mediate protein transport from ER to Golgi. Sec13 makes up lattice structure with Sec31 to form COPII vesicles. We analyzed expression of two Arabidopsis thaliana Sec13 homologs, AtSec13A and AtSec13B. AtSec13A was expressed in most parts of seedlings, while AtSec13B was partially expressed. Interaction of AtSec13A or AtSec13B with Sec31 homolog was demonstrated by bimolecular fluorescence complementation (BiFC).
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