Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 75, Issue 2, Pages 342-345Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100434
Keywords
alpha-L-arabinofuranosidase; basidiomycete; Coprinopsis cinerea; glycoside hydrolase family 62
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
Ask authors/readers for more resources
A cDNA encoding a putative extracellular alpha-L-arabinofuranosidase was cloned from the basidiomycete Coprinopsis cinerea (CcAbf62A). CcAbf62A belongs to glycoside hydrolase family 62 (GH62), but is phylogenetically distinct from previously characterized GH62 enzymes. The recombinant CcAbf62A, expressed in Pichia pastoris, released L-arabinose from both wheat arabinoxylan and oat-spelt xylan. The enzyme activity for wheat arabinoxylan was increased by the addition of CcEst1, a carbohydrate esterase from C. cinerea.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available