Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 75, Issue 7, Pages 1245-1248Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100922
Keywords
Backhousia citriodora; linalool synthase; monoterpene synthase; TPSb subfamily
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology in Japan [20780078]
- Grants-in-Aid for Scientific Research [20780078] Funding Source: KAKEN
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Using a homology-based PCR strategy, we identified a cDNA with sequence similarity to linalool synthase from lemon myrtle. Functional expression of the cDNA (designated BcLS) gene in Escherichia call yielded an active enzyme capable of catalyzing the conversion of geranyl diphosphate to (-)-linalool, i.e., an acyclic monoterpene alcohol, and to lesser amounts of cyclic monoterpenes. The kinetic parameters of BcLS were similar to those of synthases producing cyclic monoterpenes. PCR analysis revealed that the BcLS gene transcript was ubiquitously expressed in lemon myrtle and was upregulated in response to jasmonic acid treatment. Although the physiological role of neryl diphosphate (NPP) dependency of BcLS remains unclear, the cyclization activity of BcLS was enhanced when NPP was used as substrate, resulting in predominant production of cyclic monoterpenes. These findings indicate that BcLS has novel specificity and kinetic parameters, but its physiological responses to stresses such as insect damage appear to be similar to known linalool synthases.
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