Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 75, Issue 12, Pages 2283-2287Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.110379
Keywords
metagenome; siderophore; vibrioferrin; biosynthesis
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Funding
- JSPS [20810022]
- JST
- Grants-in-Aid for Scientific Research [23790134, 20810022] Funding Source: KAKEN
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A biosynthetic gene cluster of siderophore consisting of five open reading frames (ORFs) was cloned by functional screening of a metagenomic library constructed from tidal-flat sediment. Expression of the cloned biosynthetic genes in Escherichia coli led to the production of vibrioferrin, a siderophore originally reported for the marine bacterium Vibrio parahaemolyticus. To the best of our knowledge, this is the first example of heterologous production of a siderophore by biosynthetic genes cloned from a metagenomic library. The cloned cluster was one of the largest of the clusters obtained by functional screening. In this study, we demonstrated and extended the possibility of function-based metagenomic research.
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