Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 74, Issue 12, Pages 2480-2483Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100580
Keywords
neurotransmission; peptidyl prolyl cis/trans isomerase; Pin1; calcium/calmodulin-dependent protein kinase type II; phosphorylation
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To determine the role of Pint in the neurotransmission pathway, Pin1-binding proteins in mouse brain extract were identified. The Pin1-binding proteins were extracted from mouse brain homogenate, and the trypsin-digested peptides were analyzed by nano-liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins that involve the neurotransmission pathway, such as synapsin I, synapsin II, and calcium/calmodulin-dependent protein kinase type II (CaMKII), were identified in a Mascot search. Pull-down and immunoprecipitation assay indicated that Pin1 binds CaMKII in a phosphorylation-specific manner. It was assumed that Pint participates in the neurotransmission pathway involving the phosphorylation signal by CaMKII.
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