Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 74, Issue 6, Pages 1315-1319Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100184
Keywords
bialaphos resistance gene (bar); binary vector; Gateway cloning; glufosinate; plant transformation
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [16085209, 19039025, 19039014, 21024004]
- Japan Society for the Promotion of Science (JSPS) [19010675, 20570045, 21570046]
- Grants-in-Aid for Scientific Research [20570045, 16085209, 19039014, 22380005, 21024004, 21570046, 19039025] Funding Source: KAKEN
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We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA (R) was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA (R) or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.
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