Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 74, Issue 8, Pages 1722-1724Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100299
Keywords
basidiomycete; carbohydrate esterase; Coprinopsis cinerea
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Green Biomass Research
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cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase.
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