The Chloride Ion Is an Environmental Factor Affecting the Biosynthesis of Pyoluteorin and 2,4-Diacetylphloroglucinol in Pseudomonas sp YGJ3

The effects of Cl- on the antibiotics productivity of Pseudomonas sp. YGJ3 were investigated. YGJ3 produced pyoluteorin and 2,4-diacetylphloroglucinol, depending on the concentration of Cl- in the culture medium. Cl- stimulated the biosynthesis of pyoluteorin, thereby repressing 2,4-diacetylphloroglucinol biosynthesis. The cell-free extract from the bacteria grown without Cl- showed high activity of monoacetylphloroglucinol acetyltransferase, an essential enzyme in 2,4-diacetylphloroglucinol biosynthesis.