Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 5, Pages 1163-1167Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.90074
Keywords
Aspergillus nidulans; chitin synthase; myosin; actin; cell wall
Categories
Funding
- Noda institute for Scientific Research
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
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Chitin is one of the major cell wall components of ascomycete filamentous fungi, and chitin synthesis plays important roles in the morphogenesis of hyphae. In the Aspergillus nidulans genome, there are two genes, csmA and csmB, that encode a myosin motor-like domain (MMD) at their N-termini and a chitin synthase domain (CSD) at their C-termini. In our previous studies, we found that the MMD of CsmA was required for its functionality, and that CsmA and CsmB had certain overlapping functions essential for polarized filamentous growth. In this study, we constructed a strain that produced CsmB lacking the MMD (CS Delta MB). This strain exhibited defects similar to those of the csmB deletion mutant. FLAG-tagged CS Delta MB (CS Delta MB-FLAG) did not properly localize to the hyphae. CsmB was co-immunoprecipitated with actin in vivo, whereas CS Delta MB was not. These results suggest that the MMD of CsmB is crucial for its proper localization via interaction with actin.
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