Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 7, Pages 1693-1697Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.90309
Keywords
Arabidopsis thaliana; arc11; chloroplast division; plastid division
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [14760068, 17780077, 20657015, 17051023]
- Peace Nakajima Foundation
- Grants-in-Aid for Scientific Research [17780077, 17051023, 20657015, 14760068] Funding Source: KAKEN
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Symmetric chloroplast division requires a prokaryote-derived division regulator protein MinD, whose subchloroplastic localization remains to be completely established. We investigated the localization and functionality of AtMinD1 (Arabidopsis thaliana MinD) fused with a dual hemagglutinin epitope (dHA) or a yellow fluorescent protein (YFP). AtMinD1-dHA, which successfully complemented the arc11/atminD1 mutant phenotype, was predominantly located at the envelope membrane and the mid-chloroplast constriction site. Meanwhile, AtMinD1-YFP was non-functional and showed suborganellar localization partly similar to that of AtMinD1-dHA. This prompts us to reevaluate earlier transgenic and transient expression studies using fluorescent protein-tagged AtMinD1.
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