Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 5, Pages 1197-1199Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80772
Keywords
Aspergillus aculeatus; ATP-sulfurylase gene; homologous transformation; two-way selection
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Funding
- Grant-in-Aid for Young Scientists (Start-up) [18880022]
- Grants-in-Aid for Scientific Research [18880022] Funding Source: KAKEN
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A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC(+) transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.
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