Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 5, Pages 1105-1112Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80859
Keywords
methoxsalen (8-methoxy-2 ',3 ',6,7-furocoumarin); Poncirus trifoliate; Alzheimer's disease; acetylcholinesterase; amyloid beta peptide
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Funding
- Ministry of Agriculture and Forestry, Republic of Korea [106013-03-1-CG000]
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Various native Korean plants were screened to find an effective acetyleholinesterase (AChE) inhibitor for the treatment of Alzheimer's disease (AD). Among these plants, the ethanol extract of Poncirus trifoliate was selected for isolating the AChE inhibitor because it exhibited the highest inhibitory activity (47.31%). To separate the active compound from Poncirus trifoliate, solvent partition, open column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were utilized. The putative chemical structure of the AChE inhibitor was identified as methoxsalen by successive analysis with electron ionization mass spectrometry (EI-MS) and C-13/H-1-nuclear magnetic resonance (NMR). To confirm the attenuating effect of the Poncirus trifoliate extract against trimethyltin (TMT)-induced neurotoxicity, in vivo behavior tests were carried out. Our findings suggest that the Poncirus trifoliate extract significantly reversed TMT-induced learning and memory impairment. These results demonstrate that the Poncirus trifoliate extract could possess a wide range of beneficial activities for neurodegenerative disorders, notably AD.
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