Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 4, Pages 936-938Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80725
Keywords
selectable marker; nuclear transformation; acetolactate synthase; actin 2; Arabidopsis thaliana
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT)
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Most genetically modified plants are made with anti biotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.
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