Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 72, Issue 12, Pages 3273-3276Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70375
Keywords
formyltetrahydrofolate synthetase gene (fhs); reductive acetogenesis; acetogenic bacteria; methanogenesis; rumen
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A clone library of the partial formyltetrahydrofolate synthetase gene (fhs), a key enzyme in reductive acetogenesis, was constructed from the DNA of bovine rumen contents. Diverse sequences were recovered, the majority of which were clustered with the fhs of authentic acetogens. Low similarity values to known fhs were observed in all sequences, suggesting the presence of unknown acetogens.
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