Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 72, Issue 2, Pages 312-320Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70278
Keywords
Aspergillus nidulans; cellulose; induction; eglA; upstream activating sequence
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Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeR-E(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.
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