Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 72, Issue 11, Pages 3041-3043Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80428
Keywords
Arabidopsis; binary vector; selection marker; tunicamycin
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20389188]
- Feasibility Study of Japan Science, and Technology Agency
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A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.
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