Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 72, Issue 11, Pages 3021-3024Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80342
Keywords
Monascus pilosus; restriction-enzyme-mediated integration (REMI); aurintricarboxylic acid (ATA)
Categories
Funding
- National Science Council, Republic of China [NSC-95-2218-E-009-018]
- Aiming for the Top University and Elite Research Center Development plan
Ask authors/readers for more resources
The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available