Estimation of Phenolic Compounds Present in the Plant Extracts Using High Pressure Liquid Chromatography, Antioxidant Properties and its Antibacterial Activity

Objective: The aim of the present study was to evaluate total phenolic content of the prepared plant extracts and investigation of phenolic compounds present in the extract. Background: The method was done to evaluate the phenolic compounds and finding the components presence. Methods: The extraction was carried out using methanol as the solvent. Plants used in this analysis were Acalypha indica, Azadirachta indica, Lawsonia inermis and Murraya konegii. Qualitative estimation of total phenol content was done using Folin-Ciocalteu method in with gallic acid was used as standard. HPLC analysis was done to reveal the nature of phenolic compounds, C18 column was used. Antibacterial activity was carried out using agar diffusion method. Antioxidant studies were done using DPPH assay. Results: Antibacterial assay revels Lawsonia inermis showed highest zone of inhibition on Proteus sps. The high amount of phenolic content was obtained in Lawsonia inermis (5.2 mu g/ml) and the lowest was Murraya konegii (2.3 mu g/ml). HPLC of various extracts revel the presence of simple phenols, catechins, anthocyanins, aglycons, anthraquinones, glycosides form of flavones, flavonols, isoflavones and flavanones. DPPH assay revels that Lawsonia inermis has good antioxidant properties compared to other extracts.