Synthesis and characterizations of alginate-alpha-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial

A biocompatible hybrid film has been fabricated using alginate (Alg), alpha-tricalcium phosphate (alpha-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial. The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-alpha-TCP film. For this method, the Alg/alpha-TCP blend has been prepared using an ultra-sonicator and then put on a glass slide. After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness. The mechanical strength and flexibility of the film (Alg-alpha-TCP) has been controlled by changing its compositions. The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), C-13 nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses. The ATR-FTIR and C-13 NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the alpha-TCP particles reside in the network by physical interaction. The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-alpha-TCP hybrid films. The SEM analysis suggests the alpha-TCP particles are homogeneously distributed on the surface of Alg-alpha-TCP films, whereas cross-sectional images confirmed the presence of alpha-TCP in the cross-linked network. TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and alpha-TCP. The incorporation of alpha-TCP particles diminished the swelling ratio of the hybrid film. The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible. The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH7 and 7.4, and 37 degrees C. Overall, the biocompatible Alg-alpha-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.