Journal
ADVANCED BIOSYSTEMS
Volume 2, Issue 4, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adbi.201700200
Keywords
biological membranes; c-Src; extracellular vesicles; molecular recognition; supported lipid bilayers
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Funding
- CSGI (Centro per lo Sviluppo dei Sistemi a Grande Interfase-Italian Center for Colloid and Surface Science)
- project BIOMANE (Universita degli Studi di Brescia, Progetto di Ateneo Health and Wealth 2015)
- Italian Flagship program NANOMAX project N-CHEM
- SPM@ISMN facility
- CSGI
- INSTM
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Fabrication of synthetic surfaces that reproduce structure and function of biological membranes is an open challenge. This work reports the first example of supported lipid bilayers obtained from extracellular vesicles (EVSLBs). EVSLBs harness and pattern in two dimensions key properties of extracellular vesicle (EV) membranes, which present intermediate complexity between synthetic mimics and natural membranes and innate link to phenotype and function of the originating cells. Silica-supported EVSLBs are formed from nanosized EVs-separated from culture media of prostate cancer model TRAMP-C2 murine cells-following a characteristic crowding-fusion pathway. They display peculiar properties at different length scales, such as 2.5 nm roughness, lipid-raft-like domains, and cushioned patches (with the cushion filled with the native EV biomolecular cargo), which preserve the native EV membrane orientation with the proto-oncogene tyrosine-protein kinase Src(c-Src) accessible to antibody recognition.
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