Development of co-immobilized tri-enzyme biocatalytic system for one-pot pretreatment of four different perennial lignocellulosic biomass and evaluation of their bioethanol production potential

Today, many researchers are focusing on research for alternative promising energy sources and sustainable technology for bioethanol production to meet the increasing global energy demand. Here, we develop a novel one-pot pretreatment technology by co-immobilizing laccase, cellulase and beta-glucosidase to act as a tri-enzyme biocatalyst for evaluating the bioethanol production potential of four sustainable lignocellulosic biomasses viz., Typha angustifolia, Arundo donax, Saccharum arundinaceum, and Ipomoea carnea. The co-immobilized enzyme system was more stable at different temperatures and at longer storage, compared to free enzyme. During enzymatic saccharification, Saccharum arundinaceum showed higher total reducing sugar of 205 +/- 3.73 mg/g when compared to other biomass. The highest percentage of bioethanol yield of 63.43 +/- 9.35% was obtained with Ipomoea carnea. The effects of co-immobilized tri-enzyme biocatalyst on the biomasses were evaluated. The results revealed that the co-immobilized tri-enzyme biocatalyst could act as effective one-pot pretreatment for the production of bioethanol from lignocellulosic biomass.