Journal
BIORESOURCE TECHNOLOGY
Volume 269, Issue -, Pages 179-187Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.08.060
Keywords
Microalgae; Lipid; Biofuel; PEF treatment; Electroporation; Electropermeabilisation
Funding
- Helmholtz Research Program on Renewable Energies [Topic 3: Bioenergy]
- European Union's Horizon 2020 Research and Innovation program [727874]
Ask authors/readers for more resources
Pulsed Electric Field (PEF) pre-treatment, applied on fresh microalgae Auxenochlorella protothecoides, induces spontaneous release of a substantial water fraction and enables subsequent lipid extraction using ethanol-hexane blends. In this study, fresh microalgae suspensions were treated with PEF and incubated under inert conditions. Incubation promotes the release of ions and carbohydrates and increases the yields of subsequent lipid extraction thus enabling a considerable reduction of PEF-treatment energy. With a 20 h incubation period at 25 degrees C, almost total lipid extraction is achieved with a specific PEF-treatment energy of only 0.25 MJ/ kg(DW). Incubation on ice remains beneficial but less efficient than at 25 degrees C. Additionally, incubating microalgae cells in suspension at 100 g(DW)/L or in a dense paste, was almost equally efficient. Correlation between the different results suggests that spontaneous release of ions and carbohydrates facilitates more successful lipid extraction. A direct causality between the two phenomena remains to be demonstrated.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available