Journal
BIORESOURCE TECHNOLOGY
Volume 270, Issue -, Pages 430-438Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.09.059
Keywords
Clostridium saccharoperbutylacetonicum; Sucrose metabolism; Metabolic engineering; Acetone-butanol-ethanol fermentation; Sugarcane juice
Funding
- Auburn University Intramural Grants Program (IGP)
- USDA-NIFA Hatch project [ALA014-1017025]
- Alabama Agricultural Experiment Station
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy [DE-SC0015662]
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This work investigated sucrose metabolism in C. saccharoperbutylacetonicum. Inactivation of sucrose catabolism operon resulted in 28.9% decrease in sucrose consumption and 44.1% decrease in ABE production with sucrose as sole carbon source. Interestingly, a large amount of colloid-like polysaccharides were generated in the mutant, which might be due to inefficient intracellular sucrose metabolism. Deletion of transcriptional repressor gene successfully alleviated CCR and enhanced ABE production by 24.7%. Additional overexpression of endogenous sucrose pathway further elevated sucrose consumption and enhanced ABE production by 17.2%, 45.7%, or 22.5% compared to wild type with sucrose, mixed sugars or sugarcane juice as substrate, respectively. The engineered strain could be a robust platform for efficient biofuel production from inexpensive sucrose-based carbon sources.
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