Journal
BIORESOURCE TECHNOLOGY
Volume 271, Issue -, Pages 368-374Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.09.121
Keywords
Chlamydomonas reinhardtii; Biofuel; Triacylglycerol; Phospholipase; CRISPR-CAS9
Funding
- Korea CCS R&D Center (Korea CCS 2020 Project) - Korea government (Ministry of Science and ICT) [KCRC-2014M1A8A1049278]
- National Research Foundation of Korea (NRF) - Korea government [NRF-2016R1A2A1A05005465]
- Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea government (Ministry of Trade, Industry and Energy) [20172010202050]
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Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A(2) gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A(2) knockout mutants have increased by up to 64.25% (to 80.92 g L-1 d(-1)). This study can provide crucial information for the biodiesel industry.
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