Journal
BIORESOURCE TECHNOLOGY
Volume 155, Issue -, Pages 129-135Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.12.099
Keywords
Additive methane inhibition; Archaea; Cellulolytic bacteria; DGGE; qPCR
Funding
- Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2012-67015-19437]
- BOYSCAST fellowship from the Department of Science and Technology, India
- NIFA [2012-67015-19437, 578751] Funding Source: Federal RePORTER
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This study investigated the effects of saponin (0.6 g/L), nitrate (5 mM) and sulfate (5 mM), alone and in combinations, on methanogenesis, rumen fermentation, microbial community, and abundances of select microbial populations using in vitro rumen culture. Combinations of nitrate with saponin and/or sulfate additively suppressed methane production, with the lowest reduction (nearly 46%) observed for the combination of all the three inhibitors. None of the treatments adversely affected feed digestion or rumen fermentation. All the inhibitors, either alone or in combinations, did not alter the abundances of total bacteria, Ruminococcus albus, or archaea. However, saponin, alone and together with nitrate and/or sulfate, increased the abundance of Fibrobacter succinogenes and Ruminococcus flavefaciens, but decreased that of protozoa. DGGE analyses revealed limited changes in both bacterial and archaeal communities by the treatments. The nitrate-saponin-sulfate combination may be an effective and practical strategy to mitigate methane emission from ruminants. (C) 2013 Elsevier Ltd. All rights reserved.
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