Journal
BIORESOURCE TECHNOLOGY
Volume 155, Issue -, Pages 359-365Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.12.050
Keywords
Miscanthus x giganteus; Autohydrolysis; Xylooligossaccharides (XOS); Pretreatment; Degree of polymerization (DP)
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The optima conditions of production and purification of xylooligosaccharides (XOS) from Miscanthus x giganteus (MxG) were investigated. Using autohydrolysis, XOS were produced at 160, 180 and 200 degrees C at 60, 20 and 5 min, respectively. XOS yield up to 13.5% (w/w) of initial biomass and 69.2% (w/w) of xylan were achieved. Results from HPAEC-PAD analysis revealed that X1-X9 sugar oligomers were produced. Higher temperature and longer reaction time resulted in lower product molecular weight. The three optimum conditions had similar degrees of polymerization XOS. Using 10% activated carbon (w/v) with ethanol/water elution recovered 47.9% (w/w) of XOS from pretreated liquid phase. The XOS could be fractionated by degree of polymerization according to ethanol concentration in the ethanol/water elution. Most of the XOS were washed out in 30% and 50% ethanol/water (v/v) fractions. Recoveries of 91.8% xylobiose, 86.9% xylotriose, 66.3% xylotetrose, 56.2% xylopentose and 48.9% xylohexaose were observed in XOS. (C) 2014 Elsevier Ltd. All rights reserved.
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