Journal
BIORESOURCE TECHNOLOGY
Volume 161, Issue -, Pages 469-472Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.03.153
Keywords
Acid-catalyzed hot-water extraction; Microalgal lipids; Docosahexaenoic acid; Fatty acid content; Aurantiochytrium sp.
Funding
- New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea government Ministry of Knowledge Economy [20123010090010]
- Advanced Biomass R& D Center (ABC) of Global Frontier Project - Ministry of Education, Science and Technology [ABC-2012-053880]
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In this study, acid-catalyzed hot-water extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. was performed, and its yield-enhancing effects were investigated. The total fatty acid content of the Aurantiochytrium sp. was 482.5 mg/g cell, of which 141.7 mg/g cell (29.4% of total fatty acids) was DHA. The lipid-extraction yield by acid-catalyzed hot-water treatment was compared with those by organic solvents. Among the various acid-catalyzed hot-water treatment conditions, the most optimal were 1.00% H2SO4 concentration, 100 degrees C, 30 min, under which the lipid-extraction yield was 472.4 mg/g cell, and most of the DHA was extracted (29.2% of total fatty acids). Acid-catalyzed hot-water extraction treatment markedly improved the lipid-extraction yield of Aurantiochytrium sp. (C) 2014 Elsevier Ltd. All rights reserved.
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