Journal
BIORESOURCE TECHNOLOGY
Volume 158, Issue -, Pages 181-187Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.02.020
Keywords
Cellular characteristics; Benzo[a]pyrene; Copper; Stenotrophomonas maltophilia; Flow cytometry
Funding
- National Natural Science Foundation of China [U0933002, 50978122, 41330639]
- Natural Science Foundation of Guangdong Province, China [S2013020012808]
- Fundamental Research Funds for the Central Universities [2013ZM0126]
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Microbial remediation has been proposed as a promising technique to remove pollutions, however, its application has been hindered by the lack of understanding the mechanisms involved in contaminants conversion and the influence of pollutants on cellular characteristics. To address this problem, biodegradation and transformation of BaP-Cu(II) by Stenotrophomonas maltophilia, along with interactions of these pollutants with microbial cells through FCM assay were investigated. The results indicated that BaP and Cu(II) were rapidly removed by S. maltophilia on the 1st d, but only less than 10% BaP was broken down due to temporary store in cells, instead of being decomposed immediately. The key ATP enzymes in cells were then activated by BaP to promote bacteria to further decompose BaP. Stimulation of co-existed contaminants strengthened cell membrane permeability and altered cell structure, but a higher esterase activity and DNA in cells of S. maltophilia were still retained. (C) 2014 Elsevier Ltd. All rights reserved.
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