Journal
BIORESOURCE TECHNOLOGY
Volume 172, Issue -, Pages 269-275Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.09.074
Keywords
D-Lactate; Glycerol; Engineered Klebsiella pneumoniae strain; Bioconversion
Funding
- 100-Talent Project of CAS
- Natural Science Foundation of Shandong Province [ZR2013EMZ002]
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In this study, glycerol was used to produce optically pure D-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, D-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve D-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in D-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1 g/L optically pure D-lactate with a yield of 0.82 g/g glycerol, which represented the highest D-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into D-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved.
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