Journal
BIORESOURCE TECHNOLOGY
Volume 167, Issue -, Pages 324-330Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.06.035
Keywords
Vanillin; Cellulase; Inhibition kinetics; Inhibitory group
Funding
- National Natural Science Foundation of China [21306211]
- National High-Tech R&D Program of China (863 Program) [2014 AA021902]
Ask authors/readers for more resources
Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available