Journal
BIORESOURCE TECHNOLOGY
Volume 166, Issue -, Pages 620-624Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.05.098
Keywords
Chlorella vulgaris; Free fatty acid extraction; Sodium dodecyl benzene sulfonate; Esterification; Biodiesel
Funding
- New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant - Korea government Ministry of Knowledge Economy [201230 10090010]
- Advanced Biomass R&D Center (ABC) of Global Frontier Project - Ministry of Science, ICT and Future Planning [ABC-2012M3A6A2053880]
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Microalgal lipid with a high free fatty acid (FFA) content was directly extracted from Chlorella vulgaris, using SDBS, in an acid-catalyzed hot-water extraction process. The total fatty acid content of C. vulgaris was 296.0 mg/g cell. Under the 1.0% sulfuric acid, 0.4% SDBS conditions, the FFA content of the lipid increased to 96.7%, and the lipid-extraction yield was 248.4 mg/g cell. Under the 2.0% sulfuric acid, 0.2% SDBS conditions, the FFA content of the lipid was 96.1%, and the lipid-extraction yield was 266.0 mg/g cell. Whereas the FAME content of the microalgal lipid extracted by hexane-methanol was 76.4% at the 10.0% sulfuric acid concentration, the FAME content of the high-FFA microalgal lipid was increased to 70.1% at a sulfuric acid concentration of only 0.1%. By combined sulfuric acid/SDBS treatment, high-FFA microalgal lipid was extracted in large yields; moreover, the amount of catalyst was remarkably reduced in the esterification of FFA. (C) 2014 Elsevier Ltd. All rights reserved.
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