Journal
BIORESOURCE TECHNOLOGY
Volume 163, Issue -, Pages 287-294Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.04.068
Keywords
Polyhydroxyalkanoate production; Cupriavidus necator; Sodium chloride; Volatile fatty acids; Dielectric spectroscopy
Funding
- European Regional Development Fund (ERDF) Convergence Programme for the CymruH2Wales project
- ERDF-A4B for the CIRP PHA
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External stress factors in the form of ionic species or temperature increases have been shown to produce a stress response leading to enhanced PHA production. The effect of five different NaCl concentrations, namely 3.5, 6.5, 9, 12 and 15 g/l NaCl on PHA productivity using Cupriavidus necator has been investigated alongside a control (no added NaCl). A dielectric spectroscopy probe was used to measure PHA accumulation online in conjunction with the chemical offline analysis of PHA. The highest PHA production was obtained with the addition of 9 g/l NaCl, which yielded 30% higher PHA than the control. Increasing the addition of NaCl to 15 g/l was found to inhibit the production of PHA. NaCl addition can therefore be used as a simple, low cost, sustainable, non toxic and non reactive external stress strategy for increasing PHA productivity. (c) 2014 Elsevier Ltd. All rights reserved.
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