Journal
BIORESOURCE TECHNOLOGY
Volume 152, Issue -, Pages 552-556Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.11.044
Keywords
Microbial lipid; Corncob residues (CCR); Trichosporon cutaneum; Lignocellulose; Lipid titer
Funding
- National Basic Researcsh Program of China [2011CB707406]
- Natural Science Foundation of China [31300070/21306048]
- China Postdoctoral Science Foundation [2012M520850]
- Fundamental Research Funds for the Central Universities of China [WF1314036/0913005/1114054/1214025]
- State Key Laboratory of Bioreactor Engineering
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Corncob residues (CCR) are cellulose residues of corncob after xylan (hemicellulose) is extracted for production of xylitol. Here, an oleaginous yeast Trichosporon cutaneum ACCC 20271 was screened for lipid fermentation using CCR hydrolysate. The initial carbon-to-nitrogen molar ratio (C/N ratio) and the initial sugar concentration of the CCR hydrolysate were investigated in the lipid fermentation of T. cutaneum ACCC 20271. A C/N ratio gradient was generated by changing the corn steep liquor (CSL) addition and an optimal C/N ratio of 49.3 was obtained. The different initial sugar concentration was obtained by changing the cellulase amount and the lipid titer was enhanced by the increased sugar concentration. To our knowledge, this is the first report on using CCR as the feedstock for lipid fermentation. The lipid titer of 12.3 g/L and dry cell weight (DCW) of 38.4 g/L were the highest values among the studies using lignocellulose for lipid production. (C) 2013 Elsevier Ltd. All rights reserved.
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