Journal
BIORESOURCE TECHNOLOGY
Volume 151, Issue -, Pages 159-165Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.10.067
Keywords
Cellulose saccharification; Membrane reactor; Cellulase; Ionic liquids; Biofuels
Funding
- MINECO, Spain [CTQ2011-28903-C02-02]
- SENECA Foundation, Spain [08616/PI/08]
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Ultrafiltration reactors based on polymeric or ceramic membranes were shown to be suitable catalytic systems for fast enzymatic saccharification of cellulose, allowing the full recovery and reuse of enzymes. By pre-treating cellulose with the IL 1-butyl-3-methylimidazolium chloride, the suitability of this substrate for enzymatic saccharification in a reactor based on polymeric ultrafiltration membranes was demonstrated, leading to 95% cellulose hydrolysis in 4 h at 50 C. The filtration process gave a clear glucose solution (up to 113 mM) at constant permeate flow (24.7 L h(-1) m(-2)), allowing the enzyme to be reused for 9 operation cycles under semi-continuous operation, without any loss of enzyme activity. Under continuous operation mode and using ceramic ultrafiltration membranes at different residence times, the enzymatic reactor showed constant profiles in both the permeate flow rate and the glucose concentration, demonstrating the excellent suitability of the proposed approach for the saccharification of cellulose. (C) 2013 Elsevier Ltd. All rights reserved.
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