Journal
BIORESOURCE TECHNOLOGY
Volume 163, Issue -, Pages 100-105Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.04.020
Keywords
Succinic acid; Simultaneous saccharification and fermentation; Cassava; Escherichia coli NZN111
Funding
- National High Technology Research and Development Program of China [2011AA02A203, 2012AA022104, 2012AA021205]
- Fundamental Research Funds for the Central Universities [222201313007, 222201314048]
- National Special Fund for State Key Laboratory of Bioreactor Engineering [2060204]
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In this study, the production of succinic acid from cassava starch and raw cassava instead of glucose by Escherichia coli NZN111 was investigated. During the two-stage fermentation, simultaneous saccharification and fermentation (SSF) was applied in the anaerobic stage. The results showed that both the productivity and specific productivity in the process conducted at 40 degrees C were higher than those in the cultivation conducted at 37 degrees C. The yield of succinic acid based on the amount of added starch reached the highest level 0.86 g/g and cassava starch was almost totally hydrolyzed in the SSF process. With the improved cell density, 127.13 g/L of succinic acid was obtained. When the liquefied crude cassava powder was used directly in SSF, 106.17 g/L of succinic acid was formed. The result showed that crude cassava powder could be another cheap raw material for succinic acid formation. (c) 2014 Elsevier Ltd. All rights reserved.
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