Journal
BIORESOURCE TECHNOLOGY
Volume 147, Issue -, Pages 569-576Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.092
Keywords
Microalgae; Glycerol; Fed-batch; Semi-continuous; Mixotrophic growth
Funding
- General Secretariat of Universities, Research and Technology of Andalucia Government [AGR-5334]
- FEDER funds
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Mixotrophic cultures of Phaeodactylum tricornutum were carried out in bubble columns using fructose and glycerol in indoor fed-batch and semi-continuous modes. In the fed-batch cultures, different nutrient-addition strategies, combined with stepwise increments in the light intensity, were assayed. It was found that glycerol promoted significantly higher biomass productivity than fructose. A glycerol-induced photoinhibition that arrested the growth of P. tricornutun was also observed. As this was considered a limitation as regards transferring the fed-batch mode to outdoor conditions, this information was used to culture P. tricornutum in semi-continuous mode. Similar glycerol-induced photoinhibition was not observed in these cultures, even at highest dilution rates. Although the highest biomass (1.5 g L-1 d(-1)) and EPA (40 mg L-1 d(-1)) productivities found in the semi-continuous cultures were lower than those obtained photoautotrophically in outdoor photobioreactors, the findings showed that semi-continuous mode was an excellent candidate for transferring mixotrophic culture to an outdoor setting.(C) 2013 Elsevier Ltd. All rights reserved.
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