Journal
BIORESOURCE TECHNOLOGY
Volume 128, Issue -, Pages 156-163Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.098
Keywords
Biocatalysis; Co-solvent; Hesperidinase; Ionic liquid; Isoquercitrin
Funding
- Qing Lan Project of Jiangsu Province
- Science and Technology Support Program of Jiangsu Province [8E2010419]
- College Natural Science Research Project of Jiangsu Province [08KJB530002]
- Natural Science Foundation of China [21206061]
- Natural Science Foundation of Jiangsu Province [BK2009213]
- China Postdoctoral Science Foundation [2012M510124]
- Modern Agro-industry Technology Research System of China [CARS-22]
- Start Project for Introduced Talent at Jiangsu University of Science and Technology [35211002]
- Pre-research for an NSFC Project at Jiangsu University of Science and Technology [33201002]
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An ionic liquid (IL)-containing buffer system was first applied in the conversion of rutin to isoquercitrin. High substrate solubility was achieved to enhance the selectivity and efficiency of hesperidinase-catalyzed reaction. Ten ILs were selected as co-solvents to assist catalytic reactions in this biotransformation process. The transformed products of rutin were identified by LC-MS. The [Bmim][BF4]-glycine-sodium hydroxide buffer (pH 9) (10:90, v/v) was found to be the best medium for the biotransformation of isoquercitrin from rutin with higher selectivity and efficiency. The reaction time was reduced by 0.33-fold while the conversion of rutin and the yield of isoquercitrin were increased by 1.67-fold and 233-fold. The results suggest that IL co-solvents have great potential to enhance the selectively enzymatic hydrolysis of rutin for isoquercitrin production. (C) 2012 Elsevier Ltd. All rights reserved.
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