Journal
BIORESOURCE TECHNOLOGY
Volume 131, Issue -, Pages 548-551Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.01.096
Keywords
Poly(3-hydroxypropionate); Glycerol dehydratase; Propionaldehyde dehydrogenase; Glycerol-glucose cofermentation
Funding
- CAS
- Director Innovation Foundation of QI-BEBT, CAS [Y112141105]
- CAS [KSCX2-EW-G-13]
- National Natural Scientific Foundation of China [31200030]
- National 863 Project of China [SS2013AA050703-2]
- Qingdao Applied Basic Research Program [12-1-4-9-(5)-jch]
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Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. After culture condition optimization, the final engineered strain accumulated 10.1 g/L P3HP (46.4% of the cell dry weight) using glycerol and glucose as cosubstrates in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without addition of any expensive precursor. (C) 2013 Elsevier Ltd. All rights reserved.
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