Journal
BIORESOURCE TECHNOLOGY
Volume 138, Issue -, Pages 30-37Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.078
Keywords
Lipid; Fluorescence-activated cell sorting; Single cell sorting; Chlamydomonas reinhardtii; BODIPY 505/515
Funding
- Advanced Biomass R&D Center (ABC) of Korea
- Ministry of Education, Science, and Technology [ABC-2010-0029728, ABC-2012-053891]
- BK21 Post-Doctoral Research Fund
- Brain Pool Program
- Korean Federation of Science and Technology Societies Grant by Korea Government (MEST)
Ask authors/readers for more resources
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIN 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available