Journal
BIORESOURCE TECHNOLOGY
Volume 143, Issue -, Pages 238-241Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.05.050
Keywords
Lactococcus lactis; Stress tolerance; Lactic acid production; RecO
Funding
- Major State Basic Research Development Program of China (973 Program) [2010CB535014]
- Jiangnan University [JUSRP 21009]
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The aim of this study was to investigate the effect of nisin-inducible RecO expression on the stress tolerance of Lactococcus lactis NZ9000. RecO protein from Lactobacillus casei Zhang was introduced into Lactococcus lactis NZ9000 by using a nisin-inducible expression system. The recombinant strain (NZ-RecO) exhibited higher growth performances and survival rate compared with the control strain (NZ-Vector) under stress conditions. In addition, the NZ-RecO strain exhibited 1.37-, 1.41-, and 1.42-fold higher biomass, lactate production, lactate productivity, compared with the corresponding values for NZ-Vector during NaCl-stressed condition. Analysis of lactate dehydrogenase (LDH) activity showed that the production of RecO maintained the stability of LDH during salt stress. These results suggest that overproduction of RecO improved the multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 during salt stress. Results presented in this study may help to enhance the industrial utility of lactic acid bacteria. (C) 2013 Elsevier Ltd. All rights reserved.
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