Journal
BIORESOURCE TECHNOLOGY
Volume 134, Issue -, Pages 391-395Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.02.014
Keywords
Rate-limiting step; Kinetics; Microbial community ratio; Anaerobic digestion; Dairy manure
Funding
- Washington State University Agricultural Research Center
- China-US international collaborative project [2011DFA90800]
Ask authors/readers for more resources
Anaerobic digestion (AD) of complex substrates is a multi-step process, which is kinetically controlled by an individual rate-limiting step. A methodology for determining the rate-limiting step during AD of complex substrates was developed by supplementation of metabolic intermediates from each digestion step with dairy manure as an emblematic complex substrate. This method elucidated that hydrolysis of dairy manure was the rate-limiting step when normal anaerobic sludge was used as inoculum. Furthermore, the concept and effect of microbial community ratio was introduced by manipulating two different inocula, i.e. normal anaerobic sludge and heated anaerobic sludge, so that varying ratios (r) of hydrolytic and methanogenic bacteria could be studied. Results revealed that the rate-limiting step changed with the variation of r. For dairy manure, results indicated a critical ratio r* = 24 between hydrolytic bacteria and methanogens, whereby as r decreased or exceeded from this value, hydrolysis or methanogenesis limited the AD process, respectively. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available