Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 23, Issue 5, Pages 564-572Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-018-0390-6
Keywords
biosensor; 3-hydroxypropionic acid; 1; 3-propanediol; high-throughput screening; Pseudomonas denitrificans
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Funding
- Advanced Biomass R&D Center (ABC) of the Global Frontier Project [ABC-201 5M3A6A 206 611 9]
- Ministry of Science, ICT and Future Planning
- BK21-plus Program at the School of Chemical and Biomolecular Engineering at Pusan National University
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Application of the recently developed wholecell 3-HP biosensor, which detects 3-hydroxypropionic acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production.
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