Journal
BIORESOURCE TECHNOLOGY
Volume 150, Issue -, Pages 298-306Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.09.124
Keywords
Azoreductase; Brevibacillus laterosporus; Methyl orange; Immobilization; Recycle packed-bed reactor
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This study reports the identification of a new bacterial azoreductase from Brevibacillus laterosporus TISTR1911, its heterologous production in Escherichia coli, the biochemical characterization and immobilization for use in dye biodegradation processes. The recombinant azoreductase (BrAzo) is a monomeric FMN oxygen-insensitive enzyme with a molecular mass of 23 kDa showing a broad specificity for the reduction of synthetic azo dyes. Double hexahistidine-tagged BrAzo was immobilized onto a nickel chelating column and methyl orange was used to assess its degradation potential using a packed-bed reactor. The dye degradation is described by an exponential model in a downstream batchwise continuous flow mode operated with recycling. The complete degradation of methyl orange (170 mu M at 600 mL/h) was achieved in 3 h and continued over 9 cycles. Coupling the immobilized BrAzo with glucose dehydrogenase for NADH regeneration yielded a shorter 1.5 h-degradation period that was maintained throughout 16 cycles. (C) 2013 Elsevier Ltd. All rights reserved.
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